Reads with length below
N bp after demultiplexing are omitted. The default is
50. ZMWs with no reads passing are omitted.
Reads with length above
N bp are omitted for scoring in the demultiplexing step. The default is
0, meaning deactivated.
Threshold for the average barcode score of the leading and trailing ends. ZMWs with barcode score below
N are omitted. The default is
0. It is advised to set it to
26 for CLR and
80 for HiFi.
ZMWs with less than
N full passes, a read with a leading and trailing adapter, are omitted. This is only useful for CLR data. CCS / HiFi data ignore this option. The default is
0, no full-pass needed. Example
0 pass : insert - adapter - insert 1 pass : insert - adapter - INSERT - adapter - insert 2 passes: insert - adapter - INSERT - adapter - INSERT - adapter - insert