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Associate bio sample names with barcode pairs

If you want to assign a bio sample name with a barcode pair, you can use the SMRT Link feature or directly use it in lima version 2.2.0 or later.

For this, please create a CSV file with two columns Barcodes,Bio Sample. The first column contains the barcode names of the pair; if asymmetric is used, only give the pair a single name as the order of the barcodes in a pair is not relevant. Example:

Barcodes,Bio Sample
bc1001--bc1001,Alfred
bc1002--bc1002,Berthold
bc1003--bc1003,Constantin
bc1008--bc1008,Holger

Provide this CSV to lima via --biosample-csv input.csv.

This will associate the bio sample name to the read group using the SM tag.


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