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lima is the standard tool to identify barcode and primer sequences in PacBio single-molecule sequencing data. It powers the Demultiplex Barcodes, Iso-Seq, and Mark PCR Duplicates GUI-based analysis applications.

Availability

Latest version can be installed via bioconda package lima.

Please refer to our official pbbioconda page for information on Installation, Support, License, Copyright, and Disclaimer.

Latest Version

Version 2.4.0: Full changelog here

What’s new!

New documentation is up, a 1:1 port from the original GitHub docs with minor enhancements. Expect major enhancements in upcoming releases.

Get started

If you are new to demultiplexing barcoded samples, check out the Get Started guide.


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