lima is the standard tool to identify barcode and primer sequences in PacBio single-molecule sequencing data. It powers the Demultiplex Barcodes GUI-based analysis applications.
Availability
Latest version can be installed via bioconda package lima
.
Please refer to our official pbbioconda page for information on Installation, Support, License, Copyright, and Disclaimer.
Latest Version
Version 2.12.0: Full changelog here
What’s new
- Increased HiFi preset precision by improving
--single-end
- Undo demultiplexing via
lima-undo
Get started
If you are new to demultiplexing barcoded samples, check out the Get Started guide.