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lima is the standard tool to identify barcode and primer sequences in PacBio single-molecule sequencing data. It powers the Demultiplex Barcodes GUI-based analysis applications.

Availability

Latest version can be installed via bioconda package lima.

Please refer to our official pbbioconda page for information on Installation, Support, License, Copyright, and Disclaimer.

Latest Version

Version 2.13.0: Full changelog here

What’s new

Get started

If you are new to demultiplexing barcoded samples, check out the Get Started guide.

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